FREE SOLUTION CAPILLARY ELECTROPHORETIC SEPARATION OF BASIC-PROTEINS AND DRUGS USING CATIONIC POLYMER-COATED CAPILLARIES

A simple capillary coating which exhibits reversed (anodic) electroosm otic flow (EOF) has been developed for the separation of cationic comp ounds by free solution capillary electrophoresis. Poly(2-aminoethyl me thacrylate hydrochloride) (PALM) was chemically bound onto the fused-s ilica capillary inner wall. Capillaries modified by this method exhibi t an essentially pH-independent EOF in the pH range from 3 to 5.5, and have been applied to the improved separation of basic proteins and dr ugs (e.g., beta-adrenergic blocking drugs). High efficiency and precis e migration (relative standard deviation was less than 1%) were observ ed.