H. Mascher et al., DETERMINATION OF DESMETHYLSELEGILINE, METHAMPHETAMINE AND AMPHETAMINE- THE MAIN METABOLITES OF SELEGILINE IN PLASMA BY HPLC AFTER DERIVATIZATION, Journal of liquid chromatography & related technologies, 20(5), 1997, pp. 797-809
Citations number
7
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
Determination of desmethylselegiline, methamphetamine and amphetamine
in plasma requires a very sensitive and selective method. These substa
nces are metabolites of the MAO-inhibitor selegiline. This is the firs
t published HPLC method which is able to determine these three substan
ces down to a limit of quantification of 0.2 ng/mL human plasma. After
extraction as free bases into an organic solvent and reextraction as
salts into an inorganic acid all three substances and an internal stan
dard (phentermine) are derivatized for fluorescence detection after re
versed phase chromatography. The recovery is better than 90% for all s
ubstances. The linearity in the range tested (0.2 - 15 ng for each sub
stance/ml plasma) is very good indeed. The precision and accuracy is u
sually smaller than 5%. Pharmacokinetic results are presented.