THE IMAGING OF STREPTAVIDIN AND AVIDIN USING SCANNING-TUNNELING-MICROSCOPY

Authors
COOPER JM SHEN J YOUNG FM CONNOLLY P BARKER JR MOORES G
Citation
Jm. Cooper et al., THE IMAGING OF STREPTAVIDIN AND AVIDIN USING SCANNING-TUNNELING-MICROSCOPY, Journal of materials science. Materials in electronics, 5(2), 1994, pp. 106-110
Citations number
24
Categorie Soggetti
Engineering, Eletrical & Electronic","Physics, Condensed Matter","Material Science
Journal title
Journal of materials science. Materials in electronics → ACNP
ISSN journal
09574522
Volume
5
Issue
2
Year of publication
1994
Pages
106 - 110
Database
ISI
SICI code
0957-4522(1994)5:2<106:TIOSAA>2.0.ZU;2-H
Abstract
Streptavidin and avidin were adsorbed onto graphite surfaces and image s of their structures were obtained using a scanning tunnelling micros cope. Individual protein molecules and their subunits were observed, a nd their sizes were found to agree closely with measurements from a su bunit model based upon the crystal structure of streptavidin. The proc ess of adsorption of streptavidin-FITC and avidin succinylfluorescein isothiocyanate onto the graphite surface was also investigated using f luorescence microscopy.