THE ORIGIN OF TRANSFER (ORIT) OF THE ENTEROCOCCAL, PHEROMONE RESPONDING, CYTOLYSIN PLASMID PAD1 IS LOCATED WITHIN THE REPA DETERMINANT

Authors
AN FY CLEWELL DB
Citation
Fy. An et Db. Clewell, THE ORIGIN OF TRANSFER (ORIT) OF THE ENTEROCOCCAL, PHEROMONE RESPONDING, CYTOLYSIN PLASMID PAD1 IS LOCATED WITHIN THE REPA DETERMINANT, Plasmid, 37(1), 1997, pp. 87-94
Citations number
32
Categorie Soggetti
Genetics & Heredity
Journal title
Plasmid → ACNP
ISSN journal
0147619X
Volume
37
Issue
1
Year of publication
1997
Pages
87 - 94
Database
ISI
SICI code
0147-619X(1997)37:1<87:TOOT(O>2.0.ZU;2-K
Abstract
The highly conjugative pAD1 plasmid encodes a mating response to the p eptide sex pheromone cAD1 secreted by recipient strains of Enterococcu s faecalis. The location of the origin of transfer (oriT) was determin ed through the ability to mobilize the Escherichia coli-E. faecalis sh uttle plasmid pAM401 carrying specific segments of pAD1. The oriT sequ ence was found to be completely within the repA determinant known to b e required for plasmid maintenance. (C) 1997 Academic Press.