K. Fukuda et al., HYALURONIC-ACID INHIBITS INTERLEUKIN-1-INDUCED SUPEROXIDE ANION IN BOVINE CHONDROCYTES, Inflammation research, 46(3), 1997, pp. 114-117
Objective: To examine the effect of hyaluronic acid (HA) on the induct
ion of superoxide anion by IL-1 in chondrocytes. Materials and Methods
: Bovine articular chondrocytes were treated with different concentrat
ions of IL-1. A chemiluminescent probe (L-012) was added to the medium
and chemiluminescence detection was used to measure superoxide anion.
Results: IL-1 caused induction of superoxide anions in a dose-depende
nt manner. HA (10-100 mu g/ml) countered superoxide induction caused b
y 20 ng/ml of IL-1. Conclusion: HA can afford protection against carti
lage degradation, probably acting as a free-radical scavenger.