EFFECT OF CHRONIC HYPOKALEMIA ON H-K+-ATPASE EXPRESSION IN RAT COLON()

Although the kidney plays the major role in the regulation of systemic K+ homeostasis, the colon also participates substantively in K+ balan ce. The colon is capable of both K+ absorption and secretion, the magn itude of which can be modulated in response to dietary K+ intake. The H+-K+-adenosinetriphosphatase (H+-K+-ATPase) has been proposed as a po ssible mediator of K+ absorption in distal colon, but inhibitor profil es obtained in recent studies suggest that two, and perhaps more, dist inct H+-K+-ATPase activities may be present in mammalian distal colon. We have developed highly specific probes for the catalytic alpha-subu nits of colonic and gastric H+-K+-ATPase, alpha(1)-Na+-K+-ATPase, and beta-actin, which were used in Northern analysis of total RNA from who le distal colon and stomach obtained from one of three experimental gr oups of rats: 1) controls, 2) chronic dietary K+ depletion, and 3) chr onic metabolic acidosis. The probe for the colonic but not the gastric H+-K+-ATPase alpha-isoform hybridized to distal colon total RNA in al l groups. A significant increase in colonic H+-K+-ATPase mRNA abundanc e was observed in response to chronic dietary Kf depletion but not to chronic metabolic acidosis. The alpha(1)-isoform of Na+-K+-ATPase, whi ch is also expressed in distal colon, did not respond consistently to either chronic dietary K+ depletion or chronic metabolic acidosis. The gastric probe did not hybridize to total RNA from distal colon but, a s expected, hybridized to total stomach RNA. However, the abundance of gastric H+-K+-ATPase or Na+-K+-ATPase in stomach was not altered cons istently by either chronic dietary K+ depletion or metabolic acidosis. Under the conditions of this study, it appears that the mRNA encoding the colonic alpha-isoform is upregulated by chronic dietary K+ restri ction, a condition shown previously to increase K+ absorption in the d istal colon.