Y. Hano et al., SEQUENTIAL GLUCOSYLATION DETERMINED BY NMR IN THE BIOSYNTHESIS OF MULBERROSIDE-D, A CIS-OXYRESVERATROL DIGLUCOSIDE, IN MORUS-ALBA L CELL-CULTURES, Cellular and molecular life sciences, 53(3), 1997, pp. 237-241
Three new diglucosides, mulberrosides D, E, and F, were isolated from
Morus albu cell cultures and their structures were established by spec
troscopy. In the biosynthesis of mulberroside D, a cinnamoylpolyketide
-derived cis-oxyresveratrol diglucoside in the cell cultures, the diff
erent ratio of C-13-enrichment between two glucose moieties of mulberr
oside D, revealed by administering [U-C-13(6)]-D-glucose and [1,3-C-13
(2)]glycerol to the cell cultures, suggested that two glucosylation st
eps in the biosynthesis of the glucoside occur with different time cou
rses.