EVALUATION OF THE TROPHIC EFFECT OF HUMAN PLACENTAL POLYDEOXYRIBONUCLEOTIDE ON HUMAN KNEE SKIN FIBROBLASTS IN PRIMARY CULTURE

A simple assay capable of evaluating the trophic effect of growth fact ors or active principles on human skill diploid fibroblasts in primary culture has been developed. The results indicate that at physiologica l concentrations (20-100 mu g/ml) a human placental polydeoxyribonucle otide (PDRN) preparation enhances the growth of human skin diploid fib roblasts of the knee in primary culture. This effect is consistently r eproducible in the case of patients over 60 years of age, and may expl ain previously reported data on the successful clinical applications o f human placental preparations, suggesting a selective benefit of PDRN in wound healing when compared to other treatments.