R. Dolcetsanjuan et al., MICROPROPAGATION OF WALNUT TREES (JUGLANS-REGIA L) AND RESPONSE TO ARBUSCULAR MYCORRHIZAL INOCULATION, Agronomie, 16(10), 1996, pp. 639-645
Juglans regia clones were micropropagated through the establishment of
in vitro shoot-tip cultures and induction of adventitious roots. Shoo
t proliferation of juvenile J regia material was higher than with clon
es established from adult trees. Genotype and juvenility of the plant
material were determinant factors of the in vitro rooting ability. Roo
ting of adult J regia clones was improved after rejuvenation by subseq
uent subcultures. The sucrose concentration in the root elongation med
ium affected the formation of secondary roots per rooted shoot and the
acclimatization of plants. Mycorrhizal inoculation of micropropagated
walnut trees improved post-acclimatization growth in greenhouse condi
tions. Inoculation with the arbuscular mycorrhizal fungi Glomus mossea
e or G intraradices significantly improved plant survival when transfe
rred to nursery.