SOLUBLE-PROTEINS AND POLYPEPTIDE PROFILES OF SPORES OF ARBUSCULAR MYCORRHIZAL FUNGI - INTERSPECIFIC VARIABILITY AND EFFECTS OF HOST (MYC(-)) - PISUM-SATIVUM ROOT EXUDATES()) AND NONHOST (MYC()

Total soluble proteins from spores of four species of arbuscular mycor rhizal fungi (Gigaspora rosea, Scutellospora castanea, Acaulospora lae vis and Glomus mosseae) have been resolved by two-dimensional electrop horesis. Polypeptide profiles were compared to Gig rosea chosen arbitr arily as a reference. Major differences were mainly characterized by t he disappearance of some polypeptides as compared to Gig rosea. Severa l polypeptides were only detected in Gig rosea, S castanea and A laevi s. We also investigated polypeptide profiles from spores of G mosseae germinated in water or in root exudates from two host (myc(+)) genotyp es of Pisum sativum L: the genotype cv Frisson and a nodulation-defect ive mutant (P56), and from a non-host (myc(-)) mycorrhiza-resistant mu tant (P2). Although hyphal growth was weakly stimulated by the pea roo t exudates compared to water, no difference in the polypeptide pattern s was observed in extracts for spores germinated in either sterilized water or in the various pea root exudates.