THE SEQUENCE OF THE ORGYIA-PSEUDOTSUGATA MULTINUCLEOCAPSID NUCLEAR POLYHEDROSIS-VIRUS GENOME

The nucleotide sequence of the Orgyia pseudotsugata multinucleocapsid nuclear polyhedrosis virus (OpMNPV) genome was completed and analyzed. It is composed of 131,990 bases with a G + C content of 55% and conta ins 152 putative genes of 150 nucleotides or greater. Major difference s in gene content and arrangement between OpMNPV and the Autographa ca lifornica MNPV were found. These include the presence in OpMNPV of thr ee complete lap gene homologs, two conotoxin gene homologs, two protei n tyrosine phosphatase homologs, and genes encoding homologs of dUTPas e and the large and small subunits of ribonucleotide reductase. Seven major intergenic repeated regions were identified. Five of these are h omologous regions that are related to similar regions from other bacul oviruses. (C) 1997 Academic Press.