INTERLEUKIN-8 RELEASED INTO THE CEREBROSPINAL-FLUID AFTER BRAIN INJURY IS ASSOCIATED WITH BLOOD-BRAIN-BARRIER DYSFUNCTION AND NERVE GROWTH-FACTOR PRODUCTION

Citation
T. Kossmann et al., INTERLEUKIN-8 RELEASED INTO THE CEREBROSPINAL-FLUID AFTER BRAIN INJURY IS ASSOCIATED WITH BLOOD-BRAIN-BARRIER DYSFUNCTION AND NERVE GROWTH-FACTOR PRODUCTION, Journal of cerebral blood flow and metabolism, 17(3), 1997, pp. 280-289
Citations number
54
Categorie Soggetti
Neurosciences,"Endocrynology & Metabolism",Hematology
ISSN journal
0271678X
Volume
17
Issue
3
Year of publication
1997
Pages
280 - 289
Database
ISI
SICI code
0271-678X(1997)17:3<280:IRITCA>2.0.ZU;2-V
Abstract
Interleukin (IL) 8 was measured in CSF of 14 patients with severe trau matic brain injury. IL-8 levels were significantly higher in CSF (up t o 8,000 pg/ml) than serum (up to 2,400 pg/ml) (p < 0.05), suggesting i ntrathecal production. Maximal IL-8 values in CSF correlated with a se vere dysfunction of the blood-brain barrier. Nerve growth factor (NGF) was detected in CSF of 7 of 14 patients (range of maximal NGF: 62-12, 130 pg/ml). IL-8 concentrations were significantly higher in these pat ients than in those without NGF (p < 0.01), CSF containing high IL-8 ( 3,800-7,900 pg/ml) induced greater NGF production in cultured astrocyt es (202-434 pg/ml) than samples with low IL-8 (600-1,000 pg/ml), which showed a smaller NGF increase (0-165 pg/ml). Anti-IL-8 antibodies str ongly reduced (52-100%) the release of NGF in the group of high IL-8, whereas in the group with low IL-8, this effect was lower (0-52%). The inability of anti-IL-8 antibodies to inhibit the synthesis of NGF com pletely may depend on cytokines like tumor necrosis factor alpha and I L-6 found in these CSF samples, which may act in association with IL-8 . Thus, IL-8 may represent a pivotal cytokine in the pathology of brai n injury.