CHARACTERIZATION OF YELLOWFIN TUNA (THUNNUS ALBACARES, SCOMBROIDEI) TRYPTOPHAN-HYDROXYLASE

Authors
NAGAI T HAMADA M KAI N TANOUE Y NAGAYAMA F
Citation
T. Nagai et al., CHARACTERIZATION OF YELLOWFIN TUNA (THUNNUS ALBACARES, SCOMBROIDEI) TRYPTOPHAN-HYDROXYLASE, Comparative biochemistry and physiology. B. Comparative biochemistry, 116(2), 1997, pp. 161-165
Citations number
14
Categorie Soggetti
Biology
Journal title
Comparative biochemistry and physiology. B. Comparative biochemistry → ACNP
ISSN journal
03050491
Volume
116
Issue
2
Year of publication
1997
Pages
161 - 165
Database
ISI
SICI code
0305-0491(1997)116:2<161:COYT(A>2.0.ZU;2-J
Abstract
Tryptophan hydroxylase (EC 1.14.16.4) was purified from yellowfin tuna liver and properties of this enzyme were compared with those of trypt ophan hydroxylase from some other species (mouse mastocytoma and rat b rain stem). The molecular weight of the yellowfin tuna enzyme was esti mated to be about 280,000 Da. This value is similar to that for the en zymes from mouse mastocytoma and rat brain-stem. On SDS-polyacrylamide gel electrophoresis analysis, yellowfin tuna enzyme was estimated to be about 96,000 Da. This value is different from that for the enzymes from mouse mastocytoma (53,000Da) and rat brain stem (59,000Da) and su ggests that yellowfin tuna enzyme may be a dimer of identical subunits of Mr 96,000 Da. Copyright (C) 1997 Elsevier Science Inc.