Separate, extended series of positive, negative, and neutral pictures
were presented to 24 (12 men, 12 women) undergraduates. Each series wa
s presented on a different day, with full counterbalancing of presenta
tion orders. Affective state was measured using (a) orbicularis oculi
activity in response to acoustic startle probes during picture present
ation, (b) corrugator supercilii activity between and during picture p
resentation, and (c) changes in self-reports of positive and negative
affect. Participants exhibited larger eyeblink reflex magnitudes when
viewing negative than when viewing positive pictures. Corrugator activ
ity was also greater during the negative than during the positive pict
ure set, during both picture presentation and the period between pictu
res. Self-reports of negative affect increased in response to the nega
tive picture set, and self-reports of positive affect were greatest fo
llowing the positive picture set. These findings suggest that extended
picture presentation is an effective method of manipulating affective
state and further highlight the utility of startle probe and facial e
lectromyographic measures in providing on-line readouts of affective s
tate.