Two subtypes of the thromboxane A(2) (TxA(2)) receptor (TxA(2)R-E and
TxA(2)R-P), which differ in their alternatively spliced cytoplasmic ta
ils, have been identified. The initial concentration of the TxA(2) mim
etic IBOP required to reduce peak intracellular Ca2+ concentration ([C
a2+](i)) induced by a second addition of IBOP (100 nmol/L) was similar
(IC50 for TxA(2)R-E and TxA(2)R-P, 0.46+/-0.16 and 0.46+/-0.07 nnol/L
) in fibroblasts overexpressing either the TxA(2)R-E or -P subtype. Al
though the number of TxA(2) binding sites decreased in TxA(2)R-P cells
after prolonged stimulation with a TxA(2) mimetic, those in the TxA(2
)R-E cells increased markedly. To determine whether the mechanism for
desensitization differs between subtypes, the effect of activation of
protein kinase C (PKC) or cAMP-dependent kinase on TxA(2)-induced [Ca2
+](i) mobilization was measured. Forskolin reduced the IBOP-induced pe
ak [Ca2+](i) in neither TxA(2)R-E nor TxA(2)R-P cells; however, treatm
ent with phorbol esters (IC50, 0.5+/-0.70 nmol/L) strongly prevented I
BOP-mediated [Ca2+](i) rise in TxA(2)R-E but not in TxA(2)R-P cells. D
esensitization of TxA(2)R-E by phorbol eaters was prevented by the PKC
inhibitor calphostin C or by downregulation of PKC-alpha. Thus, the r
esponse of TxA(2)R-E to prolonged stimulation differs from that of TxA
(2)R-P in both the regulation of the number of binding sites and the m
echanism for desensitization; agonists that activate PKC-alpha might i
nterfere with TxA(2)R-E-mediated signaling.