EFFECTS OF ULTRAVIOLET-LIGHT ON OXY-T-9,10-EPOXY-7,8,9,10-TETRAHYDROBENZO[A]PYRENE (BPDE)-DNA ADDUCTS ADSORBED ON FILTER-PAPER AND THE DETECTION OF TETROLS BY SYNCHRONOUS SOLID-MATRIX PHOSPHORESCENCE

Exposure of BPDE-DNA adducts adsorbed on two different types of filter paper to ultraviolet radiation for 60 minutes showed that the solid-m atrix fluorescence of the adducts changed very little with time for th e adducts adsorbed on Whatman 1PS filter paper. This indicated that te trols which could be formed from the adducts were not readily produced under these conditions. Synchronous scanning solid-matrix phosphoresc ence was investigated for the detection of tetrols in the presence of BPDE-DNA adducts adsorbed on 1PS filter paper. The results showed that tetrol could be detected at sub-nanogram levels with the adducts adso rbed on the filter paper.