Dr. Gardner et al., QUANTITATIVE-ANALYSIS OF NORDITERPENOID ALKALOIDS IN LARKSPUR (DELPHINIUM SPP) BY FOURIER-TRANSFORM INFRARED-SPECTROSCOPY, Phytochemical analysis, 8(2), 1997, pp. 55-62
A Fourier transform infrared (FT-IR) spectroscopic method for measurin
g norditerpenoid alkaloids in larkspurs was developed, Alkaloids were
isolated from dry plant material using a simplified extraction procedu
re and quantitated using FT-IR. Calibration of the method was made fro
m selected larkspur samples with known alkaloid levels determined by h
igh pressure liquid chromatography and gravimetric methods. Thirty-nin
e samples from three different larkspur species (Delphinium barbeyi, D
. glaucescens, and D. occidentale) were analysed to demonstrate the ab
ility of the FT-IR method to measure toxic and total alkaloid concentr
ations in these plants. (C) 1997 by John Wiley & Sons, Ltd.