VARIOUS FRUCTOSE-1,6-BISPHOSPHATASE MESSENGER-RNAS IN MOUSE-BRAIN, LIVER, KIDNEY AND HEART

Citation
Jf. Cloix et al., VARIOUS FRUCTOSE-1,6-BISPHOSPHATASE MESSENGER-RNAS IN MOUSE-BRAIN, LIVER, KIDNEY AND HEART, NeuroReport, 8(3), 1997, pp. 617-622
Citations number
23
Categorie Soggetti
Neurosciences
Journal title
ISSN journal
09594965
Volume
8
Issue
3
Year of publication
1997
Pages
617 - 622
Database
ISI
SICI code
0959-4965(1997)8:3<617:VFMIML>2.0.ZU;2-5
Abstract
THE mouse fructose-1,6-bisphosphatase (FBPase) cDNA was previously clo ned from testicular teratocarcinoma cultured cells (F9 cells). Using t his published nucleotide sequence four primer sets were defined and us ed to amplify FBPase transcript from cerebral cortex, heart, kidney, l iver and testis of male C57B1/6 mice. Only one primer set was efficien t in all total RNA prepared from the various tissues. The restriction maps of these RNA amplification products suggested the existence of th ree different FBPase transcripts; this was confirmed by the nucleotide sequences of the FBPase transcripts and by the deduced amino acid seq uences. These data are consistent with the existence of three differen t FBPase genes. This may be relevant in neurological diseases in which abnormalities of brain glucose metabolism are involved.