VARIOUS FRUCTOSE-1,6-BISPHOSPHATASE MESSENGER-RNAS IN MOUSE-BRAIN, LIVER, KIDNEY AND HEART

THE mouse fructose-1,6-bisphosphatase (FBPase) cDNA was previously clo ned from testicular teratocarcinoma cultured cells (F9 cells). Using t his published nucleotide sequence four primer sets were defined and us ed to amplify FBPase transcript from cerebral cortex, heart, kidney, l iver and testis of male C57B1/6 mice. Only one primer set was efficien t in all total RNA prepared from the various tissues. The restriction maps of these RNA amplification products suggested the existence of th ree different FBPase transcripts; this was confirmed by the nucleotide sequences of the FBPase transcripts and by the deduced amino acid seq uences. These data are consistent with the existence of three differen t FBPase genes. This may be relevant in neurological diseases in which abnormalities of brain glucose metabolism are involved.