THE mouse fructose-1,6-bisphosphatase (FBPase) cDNA was previously clo
ned from testicular teratocarcinoma cultured cells (F9 cells). Using t
his published nucleotide sequence four primer sets were defined and us
ed to amplify FBPase transcript from cerebral cortex, heart, kidney, l
iver and testis of male C57B1/6 mice. Only one primer set was efficien
t in all total RNA prepared from the various tissues. The restriction
maps of these RNA amplification products suggested the existence of th
ree different FBPase transcripts; this was confirmed by the nucleotide
sequences of the FBPase transcripts and by the deduced amino acid seq
uences. These data are consistent with the existence of three differen
t FBPase genes. This may be relevant in neurological diseases in which
abnormalities of brain glucose metabolism are involved.