Aim. - A prospective study on bacteriological epidemiology in acute ot
its media was conducted in a pediatric hospital emergency service from
January 1993 to October 1995. Patients. - One hundred and fifty-eight
children, aged 6 months to 6 years, with an acute otitis media were i
ncluded. Culturing and cleansing of the ear canal and tympanocentesis
for aspiration and culture of the secretions were performed in 118 chi
ldren (46 of whom had received antibiotics before for 48 hours). Main
results. - Middle ear aspirates were sterile in 35% of the children wh
o had not received antibiotics and in 64% of those already treated. Ba
cteria in middle ear were predominantly Haemophilus influenzae and Str
eptococcus pneumoniae. Fifty-nine percent of S pneumoniae strains were
penicillin-resistant; however, they were responsible for clinical fai
lure in only 8% of cases. No Staphylococcus strains, commensal of the
ear canal, could be considered as pathogenic for the middle ear. Concl
usion. - The preciseness with which secretions of middle ear are aspir
ated reduces the risk of contamination and comparison of ear canal and
middle ear cultures allows to identify them. The high ratio of steril
e middle ear aspirates after antibiotic treatment raises the question
if other factors are responsible for persistent symptoms. The existenc
e of penicillin-resistant S pneumoniae must be known to adjust treatme
nt.