THE STANDARD ADDITION METHODOLOGY FOR EVALUATION OF RESULTS IN CHROMATOGRAPHIC ANALYSIS

A statistical procedure to validate a chromatographic method is descri bed. It starts by establishing the linear dynamic range (LDR) in both peak-height and peak-area, in order to choose the best instrumental si gnal for the quantitative analysis. Secondly, the performance characte ristics of the method (sensitivity, precision and detection limit) are evaluated following published criteria. The evaluation of the accurac y, assuming that a suitable CRM is not available, is performed using t he standard addition methodology (SAM). This procedure has been applie d to the determination of food preservatives in different samples.