QUANTITATIVE-ANALYSIS OF METABOLITES IN URINE BY ANTI-STOKES-RAMAN SPECTROSCOPY

Spontaneous anti-Stokes Raman spectra have been measured for urine to which glucose, acetone, or urea was added artificially, for urine incl uding glucose, acetone, and urea simultaneously, and for urine of diab etics. The anti-Stokes Raman spectra obtained are all free from the in terference from fluorescence and show a high signal-to-noise ratio. In the present system both Raman scattering from a sample and the refere nce beam from a laser are introduced into a monochromator simultaneous ly, making precise measurements of Raman intensities possible. The con centration of glucose, acetone, or urea in urine which includes one pa rticular component artificially has been determined by the intensity o f an anti-Stokes Raman band at 1130, 789, or 1016 cm(-1), respectively . The correlation coefficient (R) between the concentration of glucose , acetone, or urea and the Raman intensity has been calculated to be 0 .997, 0.96, and 0.97, respectively. The concentrations of glucose, ace tone, and urea in urine including the three components simultaneously have also been determined by the intensities of the three bands. In th is case, the R values have been found to be 0.92, 0.95, and 0.93 for g lucose, acetone, and urea, respectively. In addition, the concentratio n of glucose in urine of the diabetics has been determined by the pres ent anti-Stokes Raman system. (C) 1997 John Wiley & Sons, Inc.