THE IMMORTALIZATION OF RAT HEPATOCYTES BY TRANSFECTION WITH SV40 SEQUENCES

Authors
MACDONALD C WILLETT B
Citation
C. Macdonald et B. Willett, THE IMMORTALIZATION OF RAT HEPATOCYTES BY TRANSFECTION WITH SV40 SEQUENCES, Cytotechnology, 23(1-3), 1997, pp. 161-170
Citations number
29
Categorie Soggetti
Biothechnology & Applied Migrobiology
Journal title
Cytotechnology → ACNP
ISSN journal
09209069
Volume
23
Issue
1-3
Year of publication
1997
Pages
161 - 170
Database
ISI
SICI code
0920-9069(1997)23:1-3<161:TIORHB>2.0.ZU;2-Q
Abstract
Oncogene immortalisation has been used as an approach to isolate diffe rentiated rat liver cell lines. This paper describes the optimisation of the conditions for transfection and the characteristics of cell lin es isolated. The parenchymal origin of the immortalised lines recovere d has been demonstrated by the detection of albumin in the medium or b y bilirubin-UDP-glucuronosyl transferase activity. The lines have been characterised morphologically and expression of the cytoskeletal prot ein vimentin has been detected.