ACUTE URETERAL OBSTRUCTION AND GLOMERULOTUBULAR FUNCTION IN RATS

Urinary tract obstruction is a common cause of acute renal failure (AR F). During unilateral ureteral obstruction (UUO) arteriolar vasoconstr iction, increase in tubular pressure, and ultrafiltrate retrodiffusion occur. We studied renal function of rats with surgical UUO for 24 hr. After this period of UUO the contralateral kidney was removed and the right ureter was deobstructed. The control uninephrectomized group co nsisted of normal rats submitted to left uninephrectomy (UNx). Functio nal studies were performed 12 and 24 hr, and 7 days after deobstructio n and UNx. We measured creatinine clearance, and fractional excretion of sodium and lithium. Using conventional formulas we calculated fract ional proximal and distal sodium reabsorption. Initially we observed a reduction in glomerular filtration rate (GFR) after deobstruction (12 and 24 hr). However after 7 days, the GFR was significantly, higher i n deobstructed rats than in controls (340.3 +/- 18.3 vs. 286.4 +/- 9.3 mu L/min/100 g, p < 0.01). The dry kidney weight was also increased i n these rats. The fractional sodium excretion was increased in deobstr ucted rats, mainly in early studies (12 and 24 hr). Whereas fractional proximal reabsorption was reduced in both groups, the fractional dist al reabsorption was significantly decreased in the deobstructed group compared to UNX controls (93.9 +/- 0.9 vs. 98.9 +/- 0.1% after 24 hr, p < 0.01). Our data showed that UUO influenced both glomerular and tub ular functions. A salient finding was the overcorrection of GFR 7 days after deobstruction. The renal release of hormones and growth factors could mediate these alterations in renal function through their vascu lar, tubular, and proliferative actions.