A NOVEL IMMUNOHISTOCHEMICAL TECHNIQUE FOR THE DETECTION OF HUMAN ORALCARCINOMAS TRANSPLANTED IN NUDE-MICE USING MOUSE MONOCLONAL-ANTIBODIES

We have developed a new immunostaining technique specifically for the detection of human tumors transplanted into nude mice using mouse mono clonal antibodies (MoAbs) produced in our laboratory. The formation of a molecular complex consisting of three components (mouse MoAb or hyb ridoma supernatant, biotin-labeled anti-mouse immunoglobulins, and nor mal nude mouse serum) markedly reduced background staining and enhance d specific reaction with the transplanted tumors in nude mice. When Mo Ab production by electrofusion was screened with this new method, the incidence of hybridoma supernatant reactive with sections of transplan ted tumor was 2.3 per 100 wells immunostained. These results suggest t hat production of MoAbs using transplanted tumors is immunohistochemic ally possible and that this method may provide a new means for develop ing useful tumor markers.