ENANTIOSELECTIVE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY ASSAY OF (-ALPHA-LIPOIC AND (-)S-ALPHA-LIPOIC ACID IN HUMAN PLASMA()R)

Authors
NIEBCH G BUCHELE B BLOME J GRIEB S BRANDT G KAMPA P RAFFEL HH LOCHER M BORBE HO NUBERT I FLEISCHHAUER I
Citation
G. Niebch et al., ENANTIOSELECTIVE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY ASSAY OF (-ALPHA-LIPOIC AND (-)S-ALPHA-LIPOIC ACID IN HUMAN PLASMA()R), Chirality, 9(1), 1997, pp. 32-36
Citations number
18
Categorie Soggetti
Chemistry Medicinal","Pharmacology & Pharmacy
Journal title
Chirality → ACNP
ISSN journal
08990042
Volume
9
Issue
1
Year of publication
1997
Pages
32 - 36
Database
ISI
SICI code
0899-0042(1997)9:1<32:EHLAO(>2.0.ZU;2-A
Abstract
A specific plasma level assay for the enantiomers of a-lipoic acid is described. It makes use of liquid-liquid extraction, chemical reductio n to the dithiol enantiomers, and their precolumn chiral derivatisatio n with o-phthalaldehyde in the presence of D-phenylalanine. The two di astereomeric derivatives are separated by reversed-phase HPLC with flu orescence detection. The working range of the assay is between 15 ng/m l (lower limit of quantitation) and 1,000 ng/ml for either enantiomer. (C) 1997 Wiley-Liss, Inc.