C. Martin et al., THE USE OF RAPD MARKERS TO FACILITATE THE IDENTIFICATION OF ORYZA SPECIES WITHIN A GERMPLASM COLLECTION, Genetic resources and crop evolution, 44(2), 1997, pp. 175-183
RAPD analysis was carried out using 93 accessions held within the Oryz
a collection in the Genetic Resource Center at IRRT. These accessions
had been designated as O. meridionalis, O. glumaepatula, O. nivara or
O. rufipogon on the basis of the identification of the original collec
tor although in some cases these had been subjected to subsequent taxo
nomic revision. Following numerical analysis of the RAPD data, we prop
ose that five of the forty accessions designated as O. meridionalis an
d four of the 22 accessions designated as O. glumaepatula have been mi
s-identified. The relationship between accessions designated as O. niv
ara and O. rufipogon is complex, although it appears that some mis-ide
ntification has also occurred for these two taxa. The results indicate
that RAPD technology can be used as a fast and accurate method to ass
ist in the validation of the identification of wild Oryza species.