MOB-1, A RAS TARGET GENE, IS OVEREXPRESSED IN COLORECTAL-CANCER

Mutations in the ras oncogenes have been linked to many different canc ers, In contrast to the extensive body of knowledge related to the gen etics of ras activation, relatively little is known of the transcripti onal events triggered by ras, In previous work we have used differenti al display to identify Mob-1, a member of alpha-chemokine family, as o ne of the immediate transcriptional targets following Ras activation, Here, we provide additional experimental evidence to support this find ing by the use of an inducible H-ras expression system, the treatment of Ras farnesyl transferase inhibitor and activation of endogenous Ras by serum growth factors, We further demonstrate that IP-10, the human homolog of Mob-1, is overexpressed in the majority of colorectal canc ers.