Z. Kristofikova et J. Klaschka, IN-VITRO EFFECT OF GINKGO-BILOBA EXTRACT (EGB-761) ON THE ACTIVITY OFPRESYNAPTIC CHOLINERGIC NERVE-TERMINALS IN RAT HIPPOCAMPUS, Dementia and geriatric cognitive disorders, 8(1), 1997, pp. 43-48
The effects of Ginkgo biloba extract (EGb) applied in vitro to hippoca
mpal synaptosomes from young Wistar rats on the specific binding of [H
-3]hemicholinium-3 ([H-3]HCh-3), high-affinity choline uptake (HACU) a
nd activity of Na+,K+-ATPase were examined. EGb at a concentration of
100 mu g/ml markedly elevated the specific binding of [H-3]HCh-3 (to 3
06%) and moderately elevated HACU values (to 115%). Scatchard analysis
revealed an increase in the B-max for [H-3]HCh-3 binding, Lineweaver-
Burk analysis an increase in the V-max for choline uptake. No marked c
hanges in the activity of the sodium pump were discovered. EGb was not
able to influence the specific 'second messenger' effect of arachidon
ic acid.