SPECIFICITY OF MONOCLONAL-ANTIBODIES AGAINST ABH AND RELATED STRUCTURES TESTED BY ELISA WITH SYNTHETIC GLYCOCONJUGATES

We have tested 88 monoclonal antibodies with proposed specificities ag ainst ABH- and related antigens in an ELISA with synthetic oligosaccha ride conjugates as coating substances. All mabs were tested for bindin g towards A-, B-, and H type I trisaccharides, the B disaccharide, and the proposed anti-A and -AB were also tested on A tetrasaccharide typ e I. Of the 50 mabs with proposed A- and/or B-specificity, 28 showed a specific reaction in our ELISA. Cross-reactivity with other ABH-antig ens was observed for 7 of these 50 anti-A/B mabs and 15 of them did no t react in the ELISA. Only 2 of the 17 mabs submitted as anti-H bound to H trisaccharide type I, one of them showed a polyspecific reactivit y pattern, and the remaining 14 did not react with our type I antigen. Of the mabs with other than ABH-specificities only one, supposedly an ti-Pi, showed cross-reactivity with one of our coating antigens, in th is case the B trisaccharide.