USE OF BIOLUMINESCENCE FOR MONITORING THE VIABILITY OF INDIVIDUAL PSEUDOMONAS-PUTIDA KT2442 CELLS

Authors
EBERL L GIVSKOV M POULSEN LK MOLIN S
Citation
L. Eberl et al., USE OF BIOLUMINESCENCE FOR MONITORING THE VIABILITY OF INDIVIDUAL PSEUDOMONAS-PUTIDA KT2442 CELLS, FEMS microbiology letters, 149(1), 1997, pp. 133-140
Citations number
22
Categorie Soggetti
Microbiology
Journal title
FEMS microbiology letters → ACNP
ISSN journal
03781097
Volume
149
Issue
1
Year of publication
1997
Pages
133 - 140
Database
ISI
SICI code
0378-1097(1997)149:1<133:UOBFMT>2.0.ZU;2-P
Abstract
Exponential phase cells of Pseudomonas putida KT2442 rapidly lost viab ility when incubated at 0 degrees C without entering a viable but non- culturable state. The majority of dead cells retained their cellular i ntegrity and contained DNA. However, their cellular rRNA content was s ubstantially reduced. By employing a luciferase-marked derivative of P . putida KT2442 in combination with a highly sensitive low-light imagi ng system, live and dead cells could be distinguished.