TOXICITY OF BINARY CHEMICAL-MIXTURES - EFFECTS ON REPRODUCTION OF ZEBRAFISH (BRACHYDANIO-RERIO)

A complete life-cycle test with zebrafish was carried out with differe nt concentrations of the binary mixture 3,4-dichloroaniline and lindan e under flow-through conditions. Length and weight of fish of the F-1- generation were reduced, even in the lowest test concentration of 2 mu g/L 3,4-dichloroaniline and 40 mu g/L lindane. The same effects were found in the early life stage test for the F-2-generation. In the mixt ure of 100 mu g/L 3,4-dichloroaniline and 40 mu g/L lindane, fish whic h were exposed for their whole life time stopped spawning, irreversibl y; the fish population will become extinct. In an additional experimen t, fish were exposed to the same xenobiotic concentrations after reach ing maturity. In this case, egg production was reduced. Cessation of e gg production occurs in a concentration of 200 mu g/L 3,4-dichloroanil ine and 30 mu g/L lindane. Nevertheless, effects on spawning are influ enced by duration of exposure and the life stages of exposure.