IN-VITRO AND IN-VIVO EFFECTS OF SELENIUM AND SELENIUM WITH VITAMIN-E ON PLATELET FUNCTIONS IN DIABETIC RATS RELATIONSHIP TO PLATELET SORBITOL AND FATTY-ACID DISTRIBUTION

In vitro 30 min of incubation with selenomethionine (Sm) + vitamin E m ultiplied by about five platelet selenium (Se) decreased significantly platelet thrombin and ADP-induced aggregation decrease. Four groups o f streptozotocin-induced diabetic rats were fed with a supplemented pu rified diet with an Se-rich yeast (Selenion): DSel, Sm: DSm, Sm alpha- tocopherol: DSmE or unsupplemented diet: D. After 24 wk of supplementa tion, only a decrease in thrombin-induced aggregation in group DSel co mpared to DSm and DSmE and D was observed. However, after 24 wk of die t compared to 14 wk, in group D and DSm, a significant increase in thr ombin-induced aggregation occurred (p < 0.0001), whereas a significant decrease in groups DSel and DSmE (p < 0.0001, p < 0.03) was noted. Af ter 21 wk of diet, in DSmE, platelet adhesion to fibronectin was signi ficantly decreased compared to group D (p < 0.05). These changes in DS mE were associated with a significant decrease in platelet sorbitol (p < 0.02) and a very significant increase in platelet Se (p < 0.0005). Sm associated with vitamin E would appear more efficient to prevent ox idative damage of diabetic platelet membrane and thus to modulate its hyperactivity.