Mucor circinelloides LU M40 and Penicillium aurantiogriseum P 35, char
acterized by extracellular beta-glucosidase activity on cyanogenic gly
cosides, hydrolyse amygdalin by a two-step reaction mechanism being th
e first step of hydrolysis, from amygdalin to prunasin, very rapid (15
min) and the second one, from prunasin to mandelonitrile, much slower
(120 min).