AN INDIRECT COMPETITIVE ELISA FOR THE DETECTION OF COWS MILK AND CASEINATE IN GOATS AND EWES MILK AND CHEESE USING POLYCLONAL ANTIBODIES AGAINST BOVINE GAMMA-CASEINS

Authors
RICHTER W KRAUSE I GRAF C SPERRER I SCHWARZER C KLOSTERMEYER H
Citation
W. Richter et al., AN INDIRECT COMPETITIVE ELISA FOR THE DETECTION OF COWS MILK AND CASEINATE IN GOATS AND EWES MILK AND CHEESE USING POLYCLONAL ANTIBODIES AGAINST BOVINE GAMMA-CASEINS, ZEITSCHRIFT FUR LEBENSMITTEL-UNTERSUCHUNG UND-FORSCHUNG A-FOOD RESEARCH AND TECHNOLOGY, 204(1), 1997, pp. 21-26
Citations number
13
Categorie Soggetti
Food Science & Tenology
Journal title
ZEITSCHRIFT FUR LEBENSMITTEL-UNTERSUCHUNG UND-FORSCHUNG A-FOOD RESEARCH AND TECHNOLOGY → ACNP
ISSN journal
14314630
Volume
204
Issue
1
Year of publication
1997
Pages
21 - 26
Database
ISI
SICI code
1431-4630(1997)204:1<21:AICEFT>2.0.ZU;2-5
Abstract
An indirect competitive enzyme-linked immunosorbent assay (ELISA) meth od was developed for the detection of bovine milk and caseinate in goa ts' and ewes' milk and cheese. Polyclonal antibodies were raised in ra bbits and chickens against bovine gamma(3)-casein. In a first affinity chromatography step, antibodies recognizing caseins were absorbed on bovine casein-Sepharose. From the dialysed eluate, antibodies crossrea cting with ewes' and goats' milk protein were completely removed by im munoadsorption onto stationary phases containing ovine casein and prot ein extracted from genuine ewes' and goats' milk cheese. The detection limit of the ELISA test was 0.1% and the method was applied successfu lly during an EU collaborative study of the evaluation of methods for the detection of cows' milk.