B. Bohnert et al., DIRECT ESTERIFICATION METHOD FOR ANALYSIS OF LONG-CHAIN POLYUNSATURATED FATTY-ACIDS (LC-PUFAS) IN INFANT FORMULAS, ZEITSCHRIFT FUR LEBENSMITTEL-UNTERSUCHUNG UND-FORSCHUNG A-FOOD RESEARCH AND TECHNOLOGY, 204(1), 1997, pp. 27-31
Direct esterification of lipids in foods, including liquid and powder
infant formulae, can be used as a simplified analytical procedure for
the analysis of long-chain polyunsaturated fatty acids. The new method
gives comparable results to standard methods using lipid extraction,
but with less effort. The gas chromatographic separation method descri
bed uses an internal standard to calculate not only the normalised amo
unt of fatty acid/total fatty acids, but also the absolute amounts of
fatty acids in the product.