A FLUOROIMMUNOASSAY BASED ON IMMUNOLIPOSOMES CONTAINING GENETICALLY-ENGINEERED LIPID-TAGGED ANTIBODY

Authors
KOBATAKE E SASAKURA H HARUYAMA T LAUKKANEN ML KEINANEN K AIZAWA M
Citation
E. Kobatake et al., A FLUOROIMMUNOASSAY BASED ON IMMUNOLIPOSOMES CONTAINING GENETICALLY-ENGINEERED LIPID-TAGGED ANTIBODY, Analytical chemistry, 69(7), 1997, pp. 1295-1298
Citations number
27
Categorie Soggetti
Chemistry Analytical
Journal title
Analytical chemistry → ACNP
ISSN journal
00032700
Volume
69
Issue
7
Year of publication
1997
Pages
1295 - 1298
Database
ISI
SICI code
0003-2700(1997)69:7<1295:AFBOIC>2.0.ZU;2-4
Abstract
Immunoliposomes were prepared by using biosynthetically lipid-tagged a nti-2-phenyloxazolone single-chain antibody. Carboxyfluorescein as a f luorescent marker was encapsulated in the immunoliposomes. Some condit ions for fluoroimmunoassay using the immunoliposomes were optimized by binding assays with hapten-coated microtiter wells. A competitive flu oroimmunoassay for the caproic acid conjugate of 2-phenyloxazolone as a model antigen was performed with the immunoliposomes. In the optimiz ed assay conditions, antigen could be determined in the concentration range from 10(-7) to 10(-9) M.