THIN-LAYER CHROMATOGRAPHIC ANALYSIS OF ANTIMETABOLITES OF PYRIMIDINE-BASES IN HUMAN PLASMA

Authors
PAW B MISZTAL G
Citation
B. Paw et G. Misztal, THIN-LAYER CHROMATOGRAPHIC ANALYSIS OF ANTIMETABOLITES OF PYRIMIDINE-BASES IN HUMAN PLASMA, Chemia Analityczna, 42(1), 1997, pp. 37-40
Citations number
13
Categorie Soggetti
Chemistry Analytical
Journal title
Chemia Analityczna → ACNP
ISSN journal
00092223
Volume
42
Issue
1
Year of publication
1997
Pages
37 - 40
Database
ISI
SICI code
0009-2223(1997)42:1<37:TCAOAO>2.0.ZU;2-R
Abstract
Antimetabolites of pyrimidine bases - cytarabine, ftorafur, 6-azauridi ne, 5-fluorouracil, trifluorothymidine, and two metabolites - uracil a rabinoside (metabolite of cytarabine) and uracil (metabolite of ftoraf ur), extracted from plasma, were separated by TLC on silica gel in hor izontal chambers. The substances were visualized by UV irradiation. Af ter extraction from plasma it was possible to detect 200 ng of cytarab ine, uracil arabinoside, uracil, trifluorothymidine and 5-fluorouracil and 300 ng of ftorafur and 6-azauridine.