INDUCTION OF AMINOPEPTIDASE N CD13 ON HUMAN-LYMPHOCYTES AFTER ADHESION TO FIBROBLAST-LIKE SYNOVIOCYTES, ENDOTHELIAL-CELLS, EPITHELIAL-CELLS, AND MONOCYTES/MACROPHAGES/

Citation
D. Riemann et al., INDUCTION OF AMINOPEPTIDASE N CD13 ON HUMAN-LYMPHOCYTES AFTER ADHESION TO FIBROBLAST-LIKE SYNOVIOCYTES, ENDOTHELIAL-CELLS, EPITHELIAL-CELLS, AND MONOCYTES/MACROPHAGES/, The Journal of immunology, 158(7), 1997, pp. 3425-3432
Citations number
51
Categorie Soggetti
Immunology
Journal title
The Journal of immunology
ISSN journal
00221767 → ACNP
Volume
158
Issue
7
Year of publication
1997
Pages
3425 - 3432
Database
ISI
SICI code
0022-1767(1997)158:7<3425:IOANCO>2.0.ZU;2-0
Abstract
Aminopeptidase N (APN/CD13) is a transmembrane ectoenzyme occurring on a wide variety of cells. In contrast to monocytes and granulocytes, l ymphocytes of peripheral blood do not express CD13 Ag. However, tumor- infiltrating T cells in renal cell cancer as well as synovial fluid T cells from patients suffering from various forms of arthritis can be C D13 positive. To learn more about expression of CD13 in these tissues, we cocultured lymphocytes with different adherent cell lines. CD13 ex pression was induced in T and B lymphocytes upon adhesion to fibroblas t-like synoviocytes, HUVEC, renal tubular epithelial cells, and monocy tes/macrophages but not always upon interaction with different tumor c ell lines. Induction of APN was rapid, occurring as early as 1 h after coincubation. Expression persisted for >3 days and partially resisted inhibition by cycloheximide. Fixation of adherent cells with paraform aldehyde could not prevent induction of CD13 in lymphocytes. Soluble A PN from human kidneys or placenta could not induce CD13 expression on lymphocytes. Induction of CD13 Ag on lymphocytes required direct cell- to-cell contact as shown in experiments using dual chambers. Lymphocyt es exhibited an induction not only in CD13 protein but also in Ala-pNA -cleaving enzyme activity and in CD13 mRNA. Lymphocytic expression of CD13 represents a potentially increased cellular ability to inactivate inflammatory mediators. Furthermore, CD13 could be involved in adhesi on, in lymphocytic migration, or in the Ag processing of peptides boun d in the groove of MHC class II molecules.