K. Wuttke et al., DETECTION OF CHROMOSOME-2 AND CHROMOSOME-7 WITHIN X-RAY-INDUCED OR COLCHICINE-INDUCED MICRONUCLEI BY FLUORESCENCE IN-SITU HYBRIDIZATION, Mutagenesis, 12(2), 1997, pp. 55-59
The occurrence of chromosome 2 and chromosome 7 within micronuclei of
binucleated lymphocytes induced by X-rays or colchicine was scored usi
ng the whole chromosome painting technique. The observed frequency of
involvement in micronucleus formation was compared with the yield that
would be expected theoretically, when the random participation of eac
h chromosome is assumed, No difference was observed between the expect
ed and observed inclusion of chromosome 2 or chromosome 7 into micronu
clei after X-ray exposure (2.5 Gy). This was also the case for chromos
ome 2, but not for chromosome 7 after colchicine treatment (0.04/0.06
mu g/ml); chromosome 7 was detected approximately 1.5 times more frequ
ently in micronucleus formation than would be expected from the assump
tion of a random distribution.