SOLID-PHASE BINDING ANALYSIS OF N-CAM INTERACTIONS WITH BRAIN FODRIN

The large cytoplasmic domain form of the neural cell adhesion molecule N-CAM has been reported to interact specifically with fodrin, a subme mbranous cytoskeletal protein. We tested the abilities of fodrins from bovine brain and embryonic chicken brain to bind to N-CAM that had be en isolated from differentiated or undifferentiated mouse N2A neurobla stoma cells or from the brains of embryonic day 11 or day 14 chickens. Labeled fodrin samples bound with immobilized fodrin at a minimum sol uble fodrin concentration of 2.5.10(-3) M, but the labeled fodrin did not bind to the immobilized N-CAM when incubated at 20-fold higher fod rin concentrations.