Jy. Chun et G. Stacey, A BRADYRHIZOBIUM-JAPONICUM GENE ESSENTIAL FOR NODULATION COMPETITIVENESS IS DIFFERENTIALLY REGULATED FROM 2 PROMOTERS, Molecular plant-microbe interactions, 7(2), 1994, pp. 248-255
We report the identification and nucleotide sequence of a new symbioti
c gene (nfeC) from the soybean root nodule bacterium, Bradyrhizobium j
aponicum. A Tn5 insertion (NAD14) in this gene did not affect nitrogen
fixation but caused a significant delay in soybean nodulation. In add
ition, this mutant exhibited a reduction in its competitive ability to
nodulate soybean when coinoculated with the wild type. Sequence analy
sis of the mutated region revealed that the NAD14 Tn5 insertion mapped
within an open reading frame of 825 bp. Primer extension using B. jap
onicum mRNA from three different growth conditions, aerobic, anaerobic
, and bacteroids (i.e., symbiotic form) indicated that the upstream re
gion of the gene contained two promoters, which were differentially re
gulated in response to the growth conditions. One promoter was express
ed in bacteroids, but not under aerobic or anaerobic free-living condi
tions. The other promoter was expressed only under aerobic conditions.