ENHANCED FITNESS OF RECOMBINANT PROTEIN-SYNTHESIS IN THE STATIONARY-PHASE OF ESCHERICHIA-COLI BATCH CULTURES

The ability to synthesize both recombinant and homologous cell protein s has been studied during the stationary phase of E. coli batch cultur es. The anabolic potential of the culture dramatically decreases when entering into the stationary phase but slightly recovers several hours latter. In addition, Cl857-controlled production of beta-galactosidas e is transiently enhanced at late stages of the stationary phase. Thes e results show a non-synchronous distribution of the biosynthetic reso urces throughout culture growth phases, favouring the production of re combinant proteins in both exponentially growing and aged, stationary cells.