INCREASED THERMOSTABILITY OF 3 MESOPHILIC BETA-GALACTOSIDASES UNDER HIGH-PRESSURE

Authors
ATHES V DEGRAEVE P CAVAILLELEFEBVRE D ESPEILLAC S LEMAY P COMBES D
Citation
V. Athes et al., INCREASED THERMOSTABILITY OF 3 MESOPHILIC BETA-GALACTOSIDASES UNDER HIGH-PRESSURE, Biotechnology letters, 19(3), 1997, pp. 273-276
Citations number
16
Categorie Soggetti
Biothechnology & Applied Migrobiology
Journal title
Biotechnology letters → ACNP
ISSN journal
01415492
Volume
19
Issue
3
Year of publication
1997
Pages
273 - 276
Database
ISI
SICI code
0141-5492(1997)19:3<273:ITO3MB>2.0.ZU;2-B
Abstract
High pressure (>200 Mpa) or high temperature (>45 degrees C) can both induce an irreversible inactivation of the beta-galactosidases of Aspe rgillus oryzae, Kluyveromyces lactis and Escherichia coli. Moderate pr essures (50 MPa-250 MPa) exerted a protective effect against thermal i nactivation for the three beta-galactosidases investigated. High press ure could thus be used to carry out beta-galactosidase catalysed react ions such as lactose hydrolysis, at higher temperatures.