DETERMINATION OF OKADAIC ACID BY MICELLAR ELECTROKINETIC CHROMATOGRAPHY WITH ULTRAVIOLET DETECTION

Authors
BOUAICHA N HENNION MC SANDRA P
Citation
N. Bouaicha et al., DETERMINATION OF OKADAIC ACID BY MICELLAR ELECTROKINETIC CHROMATOGRAPHY WITH ULTRAVIOLET DETECTION, Toxicon, 35(2), 1997, pp. 273-281
Citations number
24
Categorie Soggetti
Toxicology,"Pharmacology & Pharmacy
Journal title
Toxicon → ACNP
ISSN journal
00410101
Volume
35
Issue
2
Year of publication
1997
Pages
273 - 281
Database
ISI
SICI code
0041-0101(1997)35:2<273:DOOABM>2.0.ZU;2-#
Abstract
Micellar electrokinetic chromatography (MEKC) with ultraviolet (UV) de tection was applied for the determination of non-derivatized phycotoxi ns associated with diarrhoetic shellfish poisoning. A detection limit for 40 pg of okadaic acid (OA) was achieved. The UV intensities of thi s toxin measured at 200 nm showed good linearity in the range 40-640 p g. OA was detected in mussels spiked with 10 ng/g whole tissue. The pr esence of OA and dinophysistoxin-2 was observed in the crude extract o f the dinoflagellate Prorocentrum lima. (C) 1997 Elsevier Science Ltd.