AMINO-ACID-CONCENTRATIONS IN HYPOTHALAMIC AND CAUDATE NUCLEI DURING MICROWAVE-INDUCED THERMAL-STRESS - ANALYSIS BY MICRODIALYSIS

Exposure to radiofrequency radiation (RFR) may produce thermal respons es. Extracellular amino acid concentrations in the hypothalamus (Hyp) and caudate nucleus (CN) were measured by using in vivo microdialysis before and during exposure to RFR. Under urethane anesthetic, each rat was implanted stereotaxically with a nonmetallic microdialysis probe and temperature probe guides and then placed in the exposure chamber. The rat laid on its right side with its head and neck placed directly under the wave guide. Temperature probes were placed in the left brain , right brain, face (subcutaneously), left tympanum, and rectum. Each microdialysis sample was collected over a 20 min period. The microdial ysis probe was perfused for 2 h before the rat was exposed to 5.02 GHz radiation (10 mu s pulse width, 1000 pulses/s). The right and left si des of the brain were maintained at approximately 41.2 and 41.7 degree s C, respectively, throughout a 40 min exposure period. Initially when the brain was being heated to these temperatures, the time-averaged s pecific absorption rates (SARs) for the right and left sides of the br ain were 29 and 40 W/kg, respectively. Concentrations of aspartic acid , glutamic acid, serine, glutamine, and glycine in dialysate were dete rmined by using high-pressure Liquid chromatography with electrochemic al detection. In the Hyp and CN, the concentrations of aspartic acid, serine, and glycine increased significantly during RFR exposure (P <.0 5). These results indicate that RFR-induced thermal stress produces a general change in the amino acid concentrations that is not restricted to thermoregulatory centers. Changes in the concentrations of glutami c acid (Hyp, P = .16; CN, P = .34) and glutamine (Hyp, P = .13; CN, P = .10) were not statistically significant Altered amino acid concentra tions may reveal which brain regions are susceptible to damage in resp onse to RFR-induced thermal stress. (C) 1997 Wiley-Liss, Inc.