FLUCONAZOLE TOLERANCE IN CLINICAL ISOLATES OF CRYPTOCOCCUS-NEOFORMANS

Citation
K. Venkateswarlu et al., FLUCONAZOLE TOLERANCE IN CLINICAL ISOLATES OF CRYPTOCOCCUS-NEOFORMANS, Antimicrobial agents and chemotherapy, 41(4), 1997, pp. 748-751
Citations number
24
Categorie Soggetti
Pharmacology & Pharmacy",Microbiology
ISSN journal
00664804
Volume
41
Issue
4
Year of publication
1997
Pages
748 - 751
Database
ISI
SICI code
0066-4804(1997)41:4<748:FTICIO>2.0.ZU;2-2
Abstract
Eleven isolates of Cryptococcus neoformans were investigated to determ ine the biochemical basis of their tolerance to fluconazole. The MICs of fluconazole for three isolates with low-level resistance were 3- to 6-fold higher than those for sensitive isolates, while the MICs for f our isolates with high-level resistance were 100- to 200-fold higher t han those for sensitive isolates, The level of ergosterol present in t he isolates varied, and those which had relatively low levels of ergos terol were resistant to amphotericin B, Changes in the affinity of the target enzyme (sterol 14 alpha-demethylase) and decreases in the cell ular content of fluconazole seemed to be responsible for the resistanc e in isolates with low-level and high-level resistance, respectively.