T. Kubota et al., EPITOPE REGIONS IN THE HEAVY-CHAIN OF CLOSTRIDIUM-BOTULINUM TYPE-E NEUROTOXIN RECOGNIZED BY MONOCLONAL-ANTIBODIES, Applied and environmental microbiology, 63(4), 1997, pp. 1214-1218
Seventeen monoclonal antibodies (MAbs) were previously established aga
inst the heavy chain (He) of botulinum type E neurotoxin in BALB/c mic
e immunized with the type E toroid, Five MAbs (LE15-5, LE34-6, EK19-7,
EK21-4, and AE27-9) showed toxin-neutralizing activity in mice, Two o
f the five MAbs, EK19-7 and EK21-4, recognized the regions located at
amino acid positions 731 to 787 and 811 to 897, respectively, One of t
he remaining three antibodies (LE34-6) reacted with the amino acid seq
uence VIKAIN, at amino acid positions 663 to 668, closed by the ion ch
annel-forming domain, It is suggested that the ion channel-forming dom
ain may also be associated with the blocking of acetylcholine release,
Furthermore, the amino acid sequence YLTHMRD within 30 residues of th
e C-terminal region of the He component seemed to be recognized by LE1
5-5, It has been reported that the binding domain of the type E toxin
is located on the C-terminal half of the He component, Therefore, the
neutralizing activity of LE15-5 antibody may be attributed to its abil
ity to block the binding of neurotoxin to the receptor of target cells
.