V. Pedini et al., HISTOCHEMICAL-STUDY OF LECTIN-BINDING IN THE MAJOR SALIVARY-GLANDS OFADULT FALLOW-DEER (DAMA-DAMA L), Acta histochemica, 99(1), 1997, pp. 81-89
The sugar residues in glycoconjugates present in the parotid and mandi
bular glands of the adult fallow-deer were detected and characterized
by using a battery of eight different lectin-horseradish peroxidase co
njugates. In some cases a treatment with sialidase preceded the lectin
staining. Parotid secretory cells produced glycoconjugates with N-ace
tylgalactosamine, N-acetylglucosamine and mannose residues. Mucous aci
nar cells were the most reactive sites of the mandibular gland and con
tained conspicuous quantities of oligosaccharides with terminal sialic
acid radicals. Galactosil-(beta 1 --> 3)N-acetylgalactosamine was the
most abundant penultimate sugar linked to N-acetylneuraminic acid. Ma
ndibular mucous cells also presented N-acetylglucosamine and sialylate
d components with the terminal dimer sialic acid-N-acetylgalactosamine
. Demilunar cells contained glycoconjugates with fucose and mannose re
sidues. The apical surface of duct cells was stained by all the lectin
s.