COMPARATIVE-STUDY OF THE EXPRESSION OF CELLULAR PROTEINS IN UNINFECTED AND HIV-INFECTED U937 CELLS USING 2-DIMENSIONAL SDS POLYACRYLAMIDE-GEL ELECTROPHORESIS

The cellular response to HIV infection was determined by analysing the expression of cellular proteins in uninfected and HIV-1 infected U937 cells using two-dimensional protein electrophoresis. HIV infected U93 7 cells constitute a useful model for the study of the chronic product ive infection of cells of the monocyte/macrophage lineage by the human immunodeficiency virus. Our data suggest that the expression of 70 pr oteins is modified following HIV infection: the expression of approxim ately half of these proteins was found to be increased, while that of the other half was repressed. We estimate that the expression of aroun d fifteen of these proteins was markedly changed following HIV infecti on. These results suggest that HIV infection results in the modified e xpression of approximately 0.5% of total cellular proteins. To our kno wledge, this study represents the first global quantitative analysis o f the cellular response to HIV infection in a model of chronic infecti on of cells of the monocyte-macrophage lineage. (C) 1997 Elsevier Scie nce Ireland Ltd.