ASSESSMENT OF THE GENETIC RELATEDNESS OF BARLEY ACCESSIONS (HORDEUM-VULGARE SL) RESISTANT TO SOIL-BORNE MOSAIC-INDUCING VIRUSES (BAMMV, BAYMV, BAYMV-2) USING RAPDS
F. Ordon et al., ASSESSMENT OF THE GENETIC RELATEDNESS OF BARLEY ACCESSIONS (HORDEUM-VULGARE SL) RESISTANT TO SOIL-BORNE MOSAIC-INDUCING VIRUSES (BAMMV, BAYMV, BAYMV-2) USING RAPDS, Theoretical and Applied Genetics, 94(3-4), 1997, pp. 325-330
Thirty-six Hordeum vulgare varieties and 12 H. spontaneum germplasms o
riginating from different parts of the world and showing different rea
ctions to the barley yellow mosaic virus complex (BaMMV, BaYMV, BaYMV-
2) were analyzed for genetic similarity using RAPDs. On the basis of a
n analysis of 20 selected RAPD-primers corresponding to 544 bands gene
tic similarity according to-Nei and Li (1979) was estimated to be betw
een 0.685 and 0.964. Associations between the 48 genotypes were calcul
ated using UPGMA-clustering and principal coordinate analysis. By appl
ying these methods we were able to separate H. spontaneum accessions f
rom H. vulgare varieties, and within these groups all the genotypes we
re clustered correctly according to their origin. Consequently, RAPD a
nalysis can be considered a very useful and efficient tool for the fas
t estimation of genetic relationships in barley. The correlation betwe
en genetic similarity with respect to German varieties and adaptation
of exotic barley varieties to German growing conditions is discussed.